Chris Froome won Olympic bronze with a battling performance for Team GB in the time trial.
Tour de France winner Froome completed the 54.6km course in 1:13:17 to win Team GB’s first cycling medal of the 2016 Olympic Games.
Geraint Thomas took ninth with a typically battling performance in 1:14:52.
Switzerland’s Fabian Cancellara produced a masterclass to stop the clock in 1:12:15 for his second Olympic title. Tom Dumoulin clinched silver in 1:13:02.
"I can't be disappointed with that," Froome said.
"Of course, I would have loved to have been in with a chance for gold but I gave it everything I had today and at the end of the day I've got to be happy with that.
"Fabian was the clear winner, more than a minute ahead, if I'd lost by five or six seconds, maybe I'd be disappointed with myself but Fabian was clearly the strongest guy today.
"It's an amazing feeling, the year I've had, obviously with the third Tour win and to come here and medal again for the second time in the Olympics, it's an incredible feeling."
14th for Emma Pooley in women's time trial
Team GB’s Emma Pooley finished 14th in the Rio Olympic Games women’s time trial.
The 33-year-old set a time of 46:31.98 as heavy wind and rain made for challenging conditions on the 29.9km course.
"I didn't want to just compete at another Olympics - I wanted to get a result but that's part of sport as well,” Pooley said.
"The weather was a bit different to what we expected.
"I had to ditch my visor halfway round because it got steamed up. Some people are just better at cornering in the wet, I guess."
American Kristin Armstrong won gold for a third successive time after victories in Beijing and London.
The 42-year-old stopped the clock in 44:26.42, Russia's Olga Zabelinskaya took silver in 44:31.97 with Olympic road champion Anna van der Breggen collecting bronze in a time of 44:37.80.
Coming out of retirement for the Games, 2014 Commonwealth Games silver-medallist Pooley was the ninth fastest rider in an opening 10km which included the Grumari climb.
Battling through the second time check at 19.7km, on the Grota Funda ascent, Pooley was facing a tough ask to make the podium.
The 20th fastest in the final sector, in 12:25.76 seconds, put Pooley third from the 11 riders that had crossed the line, moving down as the remainder of the field completed their rides.