Great Britain’s team pursuit Olympic champions Laura Trott, Dani King and Joanna Rowsell will ride at the IG London Nocturne elite women's criterium at Smithfield Market in London on Saturday 8 June.
The Wiggle-Honda Pro Cycling trio will compete on the streets of London, rather than the Olympic Velodrome, after their victory at the London 2012 Olympics.
"I'm really excited about this race, there are always so many fans lining the streets, and we'll want to put on a great performance."
Trott said: "I'm really excited about this race, there are always so many fans lining the streets, and we'll want to put on a great performance. The IG London Nocturne will be important for us leading into the National Championships - it will be our last real test against a competitive field."
Team manager Rochelle Gilmore added: "The IG London Nocturne will be the first time Wiggle Honda officially race as a team in the UK, in front of a massive British audience. This is a really big and important event for our team.
“We're extremely excited about racing in front of a home crowd against a strong line-up of professional women."
FACE Partnership MD James Pope, the event organiser, added: "We are committed to promoting women's cycling, and I am delighted that the Nocturne will feature the strongest women's professional team."